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Isolation and characterization of a cDNA encoding a Translin‐like protein, TRAX
Author(s) -
Aoki Katsunori,
Ishida Reiko,
Kasai Masataka
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01444-5
Subject(s) - complementary dna , isolation (microbiology) , genetics , biology , microbiology and biotechnology , gene , computational biology , bioinformatics
Translin is a DNA binding protein which specifically binds to consensus sequences at breakpoint junctions of chromosomal translocations in many cases of lymphoid malignancies. To investigate its functional significance at such recombination hotspots, we examined whether Translin interacts with other proteins using a yeast two‐hybrid system and identified an associated 33 kd protein partner, TRAX, with extensive amino acid homology. The TRAX protein was established to contain bipartite nuclear targeting sequences in its N‐terminal region, suggesting a possible role in the selective nuclear transport of Translin protein lacking any nuclear targeting motifs.