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Cloning and initial characterization of human and mouse Spot 14 genes 1
Author(s) -
Grillasca Joël-Paul,
Gastaldi Marguerite,
Khiri Hacène,
Dace Alexandra,
Peyrol Nicole,
Reynier Pascal,
Torresani Janine,
Planells Richard
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01433-0
Subject(s) - cloning (programming) , gene , microbiology and biotechnology , molecular cloning , chemistry , genetics , biology , gene expression , computer science , programming language
The intricate regulation of Spot 14 expression in rat lipogenic tissues has provided a useful tool in studying nutritional and hormonal factors involved in transcription. To gain insight into its function and its possible involvement in human lipid disorders, we cloned human and mouse Spot 14 genes that shared with the rat gene a strong homology concerning the deduced amino acid sequence (81 and 94%, respectively) as well as the promoter region. The mouse promoter was characterized by transfection studies, while quantitative RT‐PCR and in situ hybridization experiments showed that Spot 14 is expressed in human liver and, at a high level, in multiple symmetric lipomatosis nodules.