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Binding of the N‐terminal 63 kDa portion of connectin/titin to α‐actinin as revealed by the yeast two‐hybrid system
Author(s) -
Ohtsuka Hiroshi,
Yajima Hirohiko,
Maruyama Koscak,
Kimura Sumiko
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01432-9
Subject(s) - titin , sarcomere , complementary dna , yeast , myofibril , microbiology and biotechnology , biochemistry , chemistry , biology , gene , myocyte
Connectin/titin is a 3000 kDa protein which links the myosin filament to the Z‐line in vertebrate striated muscle sarcomeres. To search for the Z‐line proteins to which connectin binds, the yeast two‐hybrid system was applied using cDNA coding the N‐terminal 63 kDa fragment of connectin. Two clones coding the C‐terminal half region of α‐actinin (amino acids, 343–897 and 446–897) were obtained. Enzyme‐linked immunosorbent assay clearly demonstrated the interactions of α‐actinin and the N‐terminal 63 kDa fragment of connectin in vitro. Thus it is concluded that the N‐terminal 63 kDa portion of connectin binds to α‐actinin in the Z‐line of myofibrillar sarcomeres.