Premium
The reaction mechanism of phosphomannomutase in plants
Author(s) -
Oesterhelt Christine,
Schnarrenberger Claus,
Gross Wolfgang
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01425-1
Subject(s) - sugar , substrate (aquarium) , mannose , enzyme , yeast , chemistry , spinach , biochemistry , phosphate , stereochemistry , biology , ecology
The enzyme phosphomannomutase catalyzes the interconversion of mannose‐1‐phosphate (Man‐1‐P) and mannose‐6‐phosphate (Man‐6‐P). In mammalian cells the enzyme has to be activated by transfer of a phosphate group from a sugar‐1.6‐P 2 (Guha, S.K. and Rose, Z.B. (1985) Arch. Biochem. Biophys . 243, 168). In contrast, in the red alga Galdieria sulphuraria the co‐substrate (Man‐1.6‐P 2 or Glc‐1.6‐P 2 ) is converted to the corresponding sugar monophosphate while the substrate is converted to the sugar bisphosphate in each reaction cycle. Evidence is presented that the same reaction mechanism occurs in spinach and yeast.