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Siroheme biosynthesis in Saccharomyces cerevisiae requires the products of both the MET1 and MET8 genes
Author(s) -
Hansen Jørgen,
Muldbjerg Marianne,
Chérest Hélène,
Surdin-Kerjan Yolande
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01423-8
Subject(s) - saccharomyces cerevisiae , complementation , gene , sulfite reductase , chemistry , biochemistry , biosynthesis , genetics , biology , reductase , enzyme , phenotype
Siroheme is a uroporphyrinogen III‐derivative used by sulfite reductase as a prosthetic group. We investigated in Saccharomyces cerevisiae the possible involvement in siroheme biosynthesis of three genes, MET1 , MET8 and MET20 . The MET1 gene from S. cerevisiae was cloned and shown to be the same gene as MET20 . Sequence similitudes as well as complementation studies indicate that Met1p and Met8p are both involved in siroheme biosynthesis. In addition, we show formally that S. cerevisiae does not need vitamin B 12 for growth.