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Complementation of a yeast Δ pkc1 mutant by the Arabidopsis protein ANT
Author(s) -
Vergani Paola,
Morandini Piero,
Soave Carlo
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01399-3
Subject(s) - complementation , repressor lexa , saccharomyces cerevisiae , mutant , biology , arabidopsis , protein fragment complementation assay , bimolecular fluorescence complementation , genetics , yeast , transcription factor , lytic cycle , microbiology and biotechnology , gene , repressor , virus
The Saccharomyces cerevisiae protein kinase C homologue, PKC1, is involved in maintenance of cell integrity during polarized growth. We have used a mutant complementation approach to investigate related signal transduction pathways in higher plants. Here we report the isolation of a cDNA from Arabidopsis thaliana which partially suppresses the lytic defect of a Δ pkc1 yeast strain. The encoded protein, ANT, belongs to the AP2‐related gene family and is essential for ovule development. Expression in yeast of a LexA‐ANT fusion protein activates transcription of a reporter gene from promoters containing lexA operators. Our results support the idea that ANT acts as transcriptional activator in planta.