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Identification and expression of a variant isoform of the levocabastine‐sensitive neurotensin receptor in the mouse central nervous system
Author(s) -
Botto Jean-Marie,
Sarret Philippe,
Vincent Jean-Pierre,
Mazella Jean
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01391-9
Subject(s) - neurotensin , gene isoform , identification (biology) , central nervous system , neurotensin receptor , receptor , microbiology and biotechnology , chemistry , neuroscience , biology , neuropeptide , biochemistry , gene , botany
This work describes the molecular cloning of a variant isoform of the low‐affinity levocabastine‐sensitive neurotensin receptor isolated from mouse brain. Although the corresponding mRNA encodes for a 282 amino acid protein unable to bind neurotensin after transient transfection in COS‐7 cells, this non‐functional neurotensin receptor is expressed in cerebral neocortex, cerebellum, olfactory bulb, striatum and hypothalamus with a level similar to that of the full‐length low‐affinity neurotensin receptor. By contrast, this receptor form is very weakly expressed in mesencephalon and absent in the pituitary, but is the major product in the spinal cord.