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Co‐elevation of brain natriuretic peptide and proprotein‐processing endoprotease furin after myocardial infarction in rats
Author(s) -
Sawada Yoshie,
Inoue Masahiro,
Kanda Tsugiyasu,
Sakamaki Tetsuo,
Tanaka Shigeyasu,
Minamino Naoto,
Nagai Ryozo,
Takeuchi Toshiyuki
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01385-3
Subject(s) - furin , ventricle , brain natriuretic peptide , medicine , myocardial infarction , natriuretic peptide , heart failure , cardiology , npr2 , endocrinology , proprotein convertases , chemistry , biochemistry , enzyme , ldl receptor , cholesterol , lipoprotein
We investigated the expression of the yeast Kex2 family endoproteases furin and PACE4, and brain natriuretic peptide (BNP) in the atrium and ventricle after infarction as well as the conversion of the BNP precursor γBNP to BNP‐45. In a rat heart failure model, plasma BNP rose in two phases – first at day 3, and again at day 14. BNP mRNA, as measured by Northern blot analysis, increased strongly at day 3, then at days 14 and 28 less strongly in the atrium, and in the ventricle it increased weakly at day 3, then strongly at days 14 and 28. Furin mRNA showed the same pattern of expression as that of BNP message, whereas PACE4 message stayed unchanged after the infarction. Both furin and BNP were immunostained in the myocardium adjacent to the infarcted tissue. We suggest that after myocardial infarction, furin is co‐expressed with BNP in both the atrium and ventricle, and that furin may be responsible for the conversion of γBNP to BNP‐45.