Premium
Brain‐to‐blood active transport of β‐alanine across the blood–brain barrier
Author(s) -
Komura Junko,
Tamai Ikumi,
Senmaru Mizuho,
Terasaki Tetsuya,
Sai Yoshimichi,
Tsuji Akira
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01366-x
Subject(s) - alanine , taurine , amino acid , efflux , blood–brain barrier , leucine , biochemistry , sodium , chemistry , biology , central nervous system , endocrinology , organic chemistry
A high‐affinity antiluminal uptake system for β‐alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K t is 66.9 μM. β‐alanine uptake was energy‐, sodium‐ and chloride ion‐dependent. β‐amino acids strongly inhibited the uptake, while α‐ and γ‐amino acids had a little or no inhibitory effect. In ATP‐depleted cells, the uptake was stimulated by preloading β‐alanine or taurine but not by l ‐leucine. These results suggest that β‐alanine is actively transported across the antiluminal membrane of BCECs that is common to β‐amino acids. The system may function for the efflux from the brain to blood.