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Regulation of intracellular chloride concentration in rat lactotrophs: possible role of mitochondria
Author(s) -
Garcia L,
Rigoulet M,
Georgescauld D,
Dufy B,
Sartor P
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01365-8
Subject(s) - intracellular , chloride , mitochondrion , chemistry , biophysics , electrophysiology , patch clamp , chloride channel , metabolism , microbiology and biotechnology , biochemistry , biology , neuroscience , receptor , organic chemistry
Increasing evidence is accumulating for the involvement of chloride ions in the stimulus‐secretion coupling of pituitary cells. We show that the mean intracellular chloride concentration [Cl − ]i of rat lactotroph cells maintained in culture is high, close to 60 mM (59.4 mM), using the Cl − sensitive fluorescent probe 6‐methoxy‐ N ‐(3‐sulfopropyl) quinolinium (SPQ), coupled with whole‐cell patch‐clamp recordings. We demonstrate that this high level is correlated with the presence of mitochondrial stores of Cl − as shown by the release of Cl − in response to various metabolic inhibitors. We determine that CCP (50 μM) induces a mean [Cl − ]i increase of 15.8±5.8 mM, using combined electrophysiology and microspectrofluorimetry methods. These data strongly suggest that cell metabolism, including the mitochondrial function, modulate [Cl − ]i.