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A model for Fis N‐terminus and Fis‐invertase recognition
Author(s) -
Tzou Wen-Shyong,
Hwang Ming-Jing
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01348-8
Subject(s) - invertase , tetrapeptide , mutant , computational biology , chemistry , biochemistry , biology , gene , enzyme , peptide
In this modeling, we show that the elusive Fis N‐terminus involved in invertase‐mediated site‐specific DNA inversion is, by all indications, a hinged flapping loop. The prediction is based on a combined sequence and secondary structure alignment against known structures of protein segments, as well as a tetrapeptide fragment observed crystallographically. Its validity is strongly supported by the ability to interpret consistently the available mutagenesis data pertaining to this region including, especially, a series of deletion mutants which until this work had been a puzzle in the search for structural explanations. A model for Fis‐invertase recognition is also proposed.