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In vitro low propensity to form nucleosomes of four telomeric sequences
Author(s) -
Cacchione Stefano,
Cerone Maria Antonietta,
Savino Maria
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01318-x
Subject(s) - nucleosome , tetrahymena , eukaryote , histone octamer , histone , telomere , saccharomyces cerevisiae , dna , biology , eukaryotic chromosome fine structure , histone methylation , microbiology and biotechnology , genetics , biophysics , dna methylation , genome , gene , gene expression
The structural aspects of nucleosome assembly on telomeres are largely unknown. We analyzed by competitive reconstitution the affinities for the histone octamer of telomeric sequences from four different eukaryotic groups, Arabidopsis thaliana, mammals, Tetrahymena, and Saccharomyces cerevisiae . All telomeres reconstitute in nucleosomes with lower association constants than average nucleosomal DNA. DNase I digestion analysis suggests a multiple translational positioning and the lack of rotational positioning, probably due to telomeric repeats length (in most cases 6–8 bp), out of phase with the DNA helical repeat on the nucleosome (10.2 bp). These results could partly explain the lack of nucleosomes on lower eukaryote telomeres, and suggest a high in vivo mobility of telomeric nucleosomes.