Premium
The algorithm of estimation of the K m values for primers in DNA synthesis catalyzed by human DNA polymerase α
Author(s) -
Kolocheva Tat'yana I.,
Maksakova Galiya A.,
Zakharova Ol'ga D.,
Nevinsky Georgy A.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01298-7
Subject(s) - primer (cosmetics) , dna polymerase , dna , polymerization , dna synthesis , microbiology and biotechnology , template , catalysis , chemistry , polymerase , stereochemistry , biology , biochemistry , polymer , nanotechnology , materials science , organic chemistry
DNA synthesis with various deoxyribo homo‐ and heterooligoprimers in the presence of complementary templates was investigated. The lengthening of d(pN) n primers ( n =1–10) by a unit resulted in an increase of the primer affinity and a maximal rate of polymerization. The coefficient of the affinity enhancement of primers due to formation of one hydrogen bond between primer and template was found to be 1.35. The dependence of the primer affinity and polymerization rate on template‐primer structure in solution was analyzed and the objective laws of the changes of the KM and V max values were revealed.