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An inhibitory effect of actin on casein kinase II activity in vitro
Author(s) -
Karino Atsushi,
Tanoue Shin,
Fukuda Motoko,
Nakamura Takeshi,
Ohtsuki Kenzo
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01266-5
Subject(s) - actin , casein kinase 1 , kinase , casein kinase 2 , in vitro , chemistry , phosphorylation , actin binding protein , biochemistry , microbiology and biotechnology , protein kinase a , cyclin dependent kinase 2 , biology , actin cytoskeleton , cytoskeleton , cell
The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK‐II, A‐kinase and MAP‐kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of α‐monomeric CK‐II (CK‐IIα) as well as oligomeric CK‐II ( α 2 β 2 ) in a dose‐dependent manner, but has no effect on the activities of the two other kinases; and (ii) actin‐induced inhibition of CK‐II activity is due to the binding of actin to the α‐subunit of CK‐II and is non‐competitive with its phosphate acceptors. In addition, it is demonstrated that actin binds directly to CK‐II: both actin and CK‐II are coprecipitated by anti‐serum against Drosophila CK‐IIβ or by specific IgG against Ascaris suum muscle actin. The results presented here suggest that actin can suppress CK‐II‐mediated signal transduction.