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Molecular cloning, expression and tissue distribution of a chicken angiotensin II receptor
Author(s) -
Kempf Hervé,
Moullec Jean-Michel le,
Corvol Pierre,
Gasc Jean-Marie
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01264-1
Subject(s) - complementary dna , angiotensin ii , receptor , biology , microbiology and biotechnology , messenger rna , molecular cloning , 5 ht5a receptor , cloning (programming) , peptide sequence , homology (biology) , inositol phosphate , amino acid , endocrinology , gene , biochemistry , inositol , computer science , programming language
A cDNA encoding a chicken angiotensin II receptor from adrenal gland was isolated to serve as a molecular tool to study the role of AngII in avian embryonic development. This cDNA, sharing a high homology with another avian receptor (turkey), encodes a protein of 359 amino acids with 75% sequence identity with the mammalian type 1 receptor. Transient xpression has revealed pharmacological properties distinct from mammalian receptors and a functional coupling leading to the increase in inositol phosphate production. The AngII receptor mRNA is expressed in classical target organs for AngII (adrenal gland, heart, kidney) and, interestingly, in endothelial cells where it may mediate the peculiar vasorelaxation effect of AngII in the chicken.