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Arrest of mitochondrial biogenesis in copper‐treated sycamore cells
Author(s) -
Pádula Mário,
Aubert Serge,
Casimiro Adalcina,
Bligny Richard
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01260-4
Subject(s) - cardiolipin , fumarase , cytochrome c oxidase , mitochondrion , cytochrome , antimycin a , inner mitochondrial membrane , copper , chemistry , biochemistry , biology , microbiology and biotechnology , membrane , enzyme , phospholipid , organic chemistry
Sycamore suspension cells ( Acer pseudoplatanus L.) were grown in the presence of sublethal concentrations of copper (50 μM). During the first 5–6 days of treatment, growth was not affected, but cell respiration (coupled and uncoupled) declined to ∼60% of its normal value. This decline of respiration was attributed to a progressive diminution of the number of mitochondria in copper‐treated cells, based on the demonstration of the concomitant decline of (1) cardiolipin (diphosphatidylglycerol) and cytochrome aa 3 (cytochrome oxidase), two specific markers of mitochondrial inner membrane, and (2) fumarase activity, a specific marker of mitochondrial matrix space. In addition, the mitochondria extracted from copper‐treated cells presented the same properties as those from control cells, concerning substrate oxidation, cardiolipin and cytochrome aa 3 contents, and fumarase activity. These results strongly suggest that copper triggered an arrest of mitochondrial biogenesis, which preceded cell division arrest.