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Expression of a rapidly inactivating Ca 2+ channel in Pleurodeles oocytes during the resting season
Author(s) -
Ouadid H.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01251-3
Subject(s) - pleurodeles , chemistry , biophysics , microbiology and biotechnology , medicine , biology , amphibian , ecology
Two kinds of Ca 2+ channel activities have been recorded in unfertilized Pleurodeles oocytes during the resting season: the previously described L‐type and a transient one. The transient Ba current (I Ba‐t ) exhibited a voltage threshold of −36±7 mV, peaked at −18±8 mV and reversed around +50 mV. It showed a fast monoexponential decay with an inactivation time course of 31.4 ± 1.7 ms at −20 mV. I Ba‐t was insensitive to nifedipine and ω‐conotoxin‐GVIA but blocked by Ni 2+ (50 μM). Moreover, Cd 2+ also reduced I Ba‐t but was less efficient thane of Ni 2+ . When using Ca 2+ as the charge carrier, the amplitude and the decay of I Ca‐t were largely similar to those of I Ba‐t . These data demonstrated that the population of Ca 2+ channels could be seasonally modulated in Pleurodeles oocytes.