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Bandeiraea (Griffonia) simplicifolia lectin‐I, isolectin A 4 , reacting with Tn (GalNA αl → Ser/Thr or galabiose (Gal α 1 → 4Gal) containing ligands
Author(s) -
Albert M. Wu,
June H. Wu,
Kabat Elvin A.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01227-6
Subject(s) - lectin , glycoprotein , precipitin , griffonia simplicifolia , chemistry , glycopeptide , microbiology and biotechnology , biochemistry , concanavalin a , antibody , biology , immunology , in vitro , antibiotics
Bandeiraea (Griffonia) simplicifolia lectin‐I, isolectin A 4 (GS I‐A 4 ) reacting with the Tn(GalNAc α 1 → Ser/Thr) sequence or human blood group P k active dissacharide (E, Gal α 1 → 4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin‐inhibition assays. When human blood group P 1 or Tn active glycoproteins were tested by QPA, GS IA 4 reacted strongly with both the Tn active glycoproteins purified from asialo porcine, ovine and armadillo submandibular glands and a P 1 active glycoprotein isolated from sheep hydatid fluid. They precipitated over 80% of the lectin nitrogen added. The asialo porcine salivary glycoprotein‐GS I‐A 4 interaction was inhibited by both Tn containing glycopeptides and Gal α 1 → 4Gal indicating that GS I‐A 4 not only reacts with human blood group A(GalNAc α 1 → 3Gal) and B(Gal α 1 → 3Gal) active dissacharides, but also recognizes the Tn sequence and the E(Gal α 1 → 4Gal) ligand. From these results, the carbohydrate specificity of GS I‐A 4 can be defined as A, Tn ≥ B and E.