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Tissue‐specific expression of splice variants of the mouse voltage‐gated calcium channel α2/δ subunit
Author(s) -
Angelotti Timothy,
Hofmann Franz
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01205-7
Subject(s) - gene isoform , skeletal muscle , splice , alternative splicing , biology , microbiology and biotechnology , protein subunit , complementary dna , isozyme , cdna library , gene , genetics , biochemistry , enzyme , anatomy
Five different splice variants of mouse α2/δ subunit isoforms (α2/a‐e), which arose from various combinations of three alternatively spliced regions, were cloned with a combination of cDNA library screening and RT‐PCR. Expression patterns and relative abundance of the various isoforms in mouse tissues were determined with an RNAse protection assay. Skeletal muscle and brain expressed single isoforms, α2a and α2b, respectively; however, the cardiovascular system expressed all five isoforms. Heart expressed mainly isoforms α2c and α2d while, in contrast to other species, aorta expressed predominantly α2a, the ‘skeletal muscle’ isoform. Smooth muscle‐containing tissues expressed α2d and α2e. Thus, α2/δ isoforms are restricted in their tissue expression, suggesting an important functional role for the differentially spliced variants.