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Exogenously injected nuclear import factor p10/NTF2 inhibits signal‐mediated nuclear import and export of proteins in living cells
Author(s) -
Tachibana Taro,
Hieda Miki,
Sekimoto Toshihiro,
Yoneda Yoshihiro
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01180-5
Subject(s) - nuclear export signal , nuclear transport , microbiology and biotechnology , chemistry , nuclear localization sequence , nuclear protein , biochemistry , cell nucleus , biology , transcription factor , gene
p10/NTF2 is a cytosolic factor which is required for the translocation step in nuclear protein import in an in vitro assay with digitonin‐permeabilized cells. To study the functional roles of p10/NTF2 on protein transport between the nucleus and cytoplasm in living cells, recombinant p10/NTF2 was microinjected injo cultured mammalian cells. Cytoplasmically injected p10/NTF2 strongly inhibited the nuclear import of co‐injected NLS‐containing substrates in a dose‐dependent manner but had no effect on the diffusive import of small non‐nuclear proteins. Moreover, when injected into the cell nucleus, p10/NTF2 inhibited the nuclear export of NES‐containing substrates. The results suggest that the nuclear import factor p10/NTF2 may also be involved in the nuclear export of proteins and that the protein transport efficiency between the nucleus and cytoplasm may be regulated by the intracellular level of p10/NTF2.