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ATF site of human RB gene promoter is a responsive element of myogenic differentiation
Author(s) -
Okuyama Yusuke,
Sowa Yoshihiro,
Fujita Tsuyoshi,
Mizuno Takakazu,
Nomura Hitoshi,
Nikaido Toshio,
Endo Takeshi,
Sakai Toshiyuki
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01178-7
Subject(s) - microbiology and biotechnology , electrophoretic mobility shift assay , promoter , point mutation , chemistry , promoter activity , plasmid , response element , transfection , luciferase , gene , mutation , biology , transcription factor , gene expression , biochemistry
RB mRNA increases during terminal differentiation of C2 myoblasts. We demonstrate that RB promoter activity increases about 4‐fold during differentiation. The increase of RB promoter activity was reduced when a point mutation was designed in the ATF site. In a gel shift assay of the ATF site, two specific bands were observed. One of them, with the lower mobility, disappeared during differentiation. This band reacted with an antibody against ATF‐1. We cotransfected an RB promoter‐luciferase plasmid with the TREB36/ATF‐1 plasmid. ATF‐1 suppressed the activity of the wild‐type RB promoter but not of that with a point mutation at the ATF site. These results suggest that the ATF site of the RB promoter is a responsive element during myogenic differentiation of C2 cells. We hypothesize that RB promoter activity is stimulated partially due to the dissociation of ATF‐1, which suppresses the promoter activity through the ATF site in C2 myoblasts.