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Different efficiencies of the Tag and AlkA DNA glycosylases from Escherichia coli in the removal of 3‐methyladenine from single‐stranded DNA
Author(s) -
Bjelland Svein,
Seeberg Erling
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01166-0
Subject(s) - dna glycosylase , dna , escherichia coli , base excision repair , dna repair , biochemistry , dna replication , biology , chemistry , microbiology and biotechnology , gene
Escherichia coli possesses two different DNA repair glycoselases, Tag and AlkA, which have similar ability to remove the alkylation product 3‐methyladenine from double‐stranded DNA. In this study we show that these enzymes have quite different activities for the excision of 3‐methyladenine from single‐stranded DNA, AlkA being 10–20 times more efficient than Tag. We propose that AlkA and perhaps other glycosylases as well may have an important role in the excision of base damage from single‐stranded regions transiently formed in DNA during transcription and replication.