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Characterization of a human gene related to genes encoding somatostatin receptors
Author(s) -
Kolakowski Lee F.,
Jung Benjamin P.,
Nguyen Tuan,
Johnson Michael P.,
Lynch Kevin R.,
Cheng Regina,
Heng Henry H.Q.,
George Susan R.,
O'Dowd Brian F.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01160-x
Subject(s) - biology , somatostatin receptor 1 , gene , complementary dna , open reading frame , microbiology and biotechnology , coding region , somatostatin receptor , somatostatin receptor 2 , expressed sequence tag , genetics , somatostatin receptor 3 , cdna library , g protein coupled receptor , untranslated region , peptide sequence , receptor , messenger rna
We report the identification of a gene, named SLC‐1 1 , encoding a novel G protein‐coupled receptor (GPCR). A customized search procedure of a database of expressed sequence tags (dbEST) retrieved a human cDNA sequence that partially encoded a GPCR. A genomic DNA fragment identical to the cDNA was obtained and used to screen a library to isolate the full‐length coding region of the gene. This gene was intronless in its open reading frame, and encoded a receptor of 402 amino acids, and shared −40% amino acid identity in the transmembrane (TM) regions to the five known human somatostatin receptors. Northern blot analysis revealed that SLC‐1 is expressed in human brain regions, including the forebrain and hypothalamus. Expression in the rat was highest in brain, followed by heart, kidney, and ovary. Expression of SLC‐1 in COS‐7 cells failed to show specific binding to radiolabelled Tyr 1 ‐somatostatin‐14, naloxone, bremazocine, 1,3‐di(2‐tolyl)‐guanidine (DTG), or haloperidol. A repeat polymorphism of the form (CA) n was discovered in the 5′‐untranslated region (UTR) of the gene and SLC‐1 was mapped to chromosome 22, q13.3.