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Analysis of connexin43 gene expression induced by retinoic acid in F9 teratocarcinoma cells
Author(s) -
Clairmont Annette,
Tessmann Dirk,
Sies Helmut
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01129-5
Subject(s) - teratocarcinoma , retinoic acid , microbiology and biotechnology , chemistry , gene expression , gene , biology , biochemistry , cellular differentiation
All‐trans retinoic acid (10 −7 M) induces cell‐cell communication and expression of the gap junction protein connexin43 in mouse F9 teratocarcinoma cells. Northern blot analysis revealed an increase of connexin43 mRNA after treatment with retinoic acid, accompanied by an increase of the mRNA of collagen IV, a differentiation marker. To address the question at what level gene expression is enhanced by retinoic acid, nuclear run‐on experiments were carried out. There was no detectable change in the level of newly transcribed connexin43 mRNA. Therefore, we postulate a post‐transcriptional mechanism responsible for the regulation of connexin43 mRNA levels by retinoic acid.