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hNRAGE, a human neurotrophin receptor interacting MAGE homologue, regulates p53 transcriptional activity and inhibits cell proliferation
Author(s) -
Wen Chuan-Jun,
Xue Bin,
Qin Wen-Xin,
Yu Mingyan,
Zhang Min-Yue,
Zhao Dong-Hong,
Gao Xiang,
Gu Jian-Ren,
Li Chao-Jun
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00353-9
Subject(s) - microbiology and biotechnology , cell growth , cell cycle , neurotrophin , cell cycle checkpoint , biology , embryonic stem cell , phosphorylation , receptor , low affinity nerve growth factor receptor , cell , function (biology) , gene , biochemistry
hNRAGE, a neurotrophin receptor p75 interacting MAGE homologue, is cloned from a human placenta cDNA library. hNRAGE can inhibit the colony formation of and arrest cell proliferation at the G1/S and G2/M stages in hNRAGE overexpressing cells. Interestingly, hNRAGE also increases the p53 protein level as well as its phosphorylation (Ser392). Further studies demonstrated that hNRAGE does not affect the proliferation of mouse p53−/− embryonic fibroblasts, suggesting that p53 function is required for hNRAGE induced cell cycle arrest. Moreover, the cell cycle inhibiting protein p21 WAF is induced by hNRAGE in a p53 dependent manner. The data provide original evidence that hNRAGE arrests cell growth through a p53 dependent pathway.