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Sp1 is involved in the transcriptional activation of p16 INK4 by p21 Waf1 in HeLa cells
Author(s) -
Xue Lixiang,
Wu Junfeng,
Zheng Wenjie,
Wang Peichang,
Li Jun,
Zhang Zongyu,
Tong Tanjun
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00352-7
Subject(s) - biology , transcription (linguistics) , hela , transcription factor , downregulation and upregulation , cell cycle , microbiology and biotechnology , cancer research , physics , cell , genetics , gene , philosophy , linguistics
Both p16 INK4 and p21 Waf1 are very important negative regulators of the cell cycle. In this study we examined the effects of p21 Waf1 on the transcription of p16 INK4 . We determined that p21 Waf1 can activate the transcription of p16 INK4 , and that this effect is GC‐box dependent. We also found that the transcription factor Sp1 plays a key role in this event. Upregulation of Sp1 contributes to the transcriptional activation and protein level of p16 INK4 mediated by p21 Waf1 , and is a potential point of cooperation between the p16/pRb and p14 (ARF)/p53 tumor suppressor pathways.