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P66 ShcA interacts with MAPKAP kinase 2 and regulates its activity
Author(s) -
Yani Yvonne M.,
Gaestel Matthias,
Lin Lih-Ling
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00351-5
Subject(s) - microbiology and biotechnology , immunoprecipitation , signal transducing adaptor protein , chemistry , protein kinase a , kinase , phosphorylation , signal transduction , biochemistry , biology , gene
Three mitogen activated protein kinase‐activated protein kinase 2 (MAPKAP kinase 2, MK2) interacting proteins were identified using a yeast two‐hybrid approach. ShcA, a signaling phospho‐protein, human polyhomeotic 2 (HPH2), a transcriptional regulator, and highly similar to smoothelin (HSTS), which is related to the cytoskeletal associated protein smoothelin, interact specifically with MK2. The interaction of MK2 with the 66 kDa isoform of ShcA, p66 ShcA , and HPH2 was confirmed using co‐immunoprecipitation. MK2 is activated with p66 ShcA co‐expression and p66 ShcA is an in vitro substrate for MK2, further demonstrating their association and suggesting a biological role for p66 Shc in MK2 activation.