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FAD assembly and thylakoid membrane binding of ferredoxin:NADP + oxidoreductase in chloroplasts
Author(s) -
Onda Yayoi,
Hase Toshiharu
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00325-4
Subject(s) - thylakoid , oxidoreductase , chloroplast , ferredoxin—nadp(+) reductase , ferredoxin , flavin adenine dinucleotide , flavin group , biochemistry , membrane , biosynthesis , chemistry , biology , biophysics , cofactor , enzyme , gene
We investigated the process of flavin adenine dinucleotide (FAD) incorporation into the ferredoxin (Fd):NADP + oxidoreductase (FNR) polypeptide during FNR biosynthesis, using pull‐down assay with resin‐immobilized Fd which bound strongly to FAD‐assembled holo‐FNR, but hardly to FAD‐deficient apo‐FNR. After FNR precursor was imported into isolated chloroplasts and processed to the mature size, the molecular form pulled down by Fd‐resin increasingly appeared. The mature‐sized FNR (mFNR) accumulated transiently in the stroma as the apo‐form, and subsequently bound on the thylakoid membranes as the holo‐form. Thus, FAD is incorporated into the mFNR inside chloroplasts, and this assembly process is followed by the thylakoid membrane localization of FNR.