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In vivo 13 C‐NMR and modelling study of metabolic yield response to ethanol stress in a wild‐type strain of Saccharomyces cerevisiae
Author(s) -
Martini Silvia,
Ricci Maso,
Bonechi Claudia,
Trabalzini Lorenza,
Santucci Annalisa,
Rossi Claudio
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00316-3
Subject(s) - saccharomyces cerevisiae , ethanol , yield (engineering) , in vivo , chemistry , biochemistry , nuclear magnetic resonance spectroscopy , biophysics , yeast , stereochemistry , biology , thermodynamics , physics , microbiology and biotechnology
In this paper the combined use of in vivo 13 C‐nuclear magnetic resonance spectroscopy and mathematical modelling allowed the analysis of the response to ethanol stress in a wild‐type strain of Saccharomyces cerevisiae , in terms of a reduced metabolic activity. The model developed succeeded in describing and interpreting the effects of increasing concentrations of exogenous ethanol. In particular, the ratio between the kinetic constants associated with ethanol production and glucose consumption gave the estimation of the metabolic yield of the processes in perfect agreement with experimental results.