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Adenine nucleotide translocase 3 (ANT3) overexpression induces apoptosis in cultured cells
Author(s) -
Zamora Mònica,
Granell Meritxell,
Mampel Teresa,
Viñas Octavi
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00293-5
Subject(s) - apoptosis , adenine nucleotide translocator , hela , biology , mitochondrial apoptosis induced channel , microbiology and biotechnology , mitochondrion , annexin , transfection , gene isoform , adenine nucleotide , caspase 9 , caspase , programmed cell death , biochemistry , in vitro , nucleotide , gene
Mitochondrial adenine nucleotide translocase 1 (ANT1), but not ANT2, can dominantly induce apoptosis [Bauer et al. (1999) J. Cell Biol. 439, 258–262]. Nothing is known, however, about the apoptotic activity of ANT3. We have transfected HeLa cells with the three human ANT isoforms to compare their potential as inducers of apoptosis. Transient overexpression of ANT3 resulted, like ANT1, in apoptosis as shown by an increase in the sub‐G1 fraction, annexin V staining, low Δ Ψ m , and activation of caspases 9 and 3. Moreover, the apoptosis produced by ANT3 was inhibited by bongkrekic acid and by cyclosporin A. The pro‐apoptotic activities of the ANT1 and ANT3 isoforms contrast with the lack of apoptotic activity of ANT2. This finding may help to identify the specific factors associated with the pro‐apoptotic activities of ANT isoforms.