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The inhibition of cell spreading on a cellulose substrate (cuprophan) induces an apoptotic process via a mitochondria‐dependent pathway
Author(s) -
Gekas Jean,
Hindié Mathilde,
Faucheux Nathalie,
Lanvin Olivia,
Mazière Cécile,
Fuentès Vincent,
Gouilleux-Gruart Valérie,
David Bertrand,
Mazière Jean-Claude,
Lassoued Kaı̈ss,
Nagel Marie-Danielle
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00276-5
Subject(s) - apoptosis , phosphatidylserine , microbiology and biotechnology , mitochondrion , cellulose , chemistry , cell , programmed cell death , caspase , biophysics , membrane , biochemistry , biology , phospholipid
Cell shape was found to be a strong indicator of whether individual cells grow or die, and may play an important role in controlling apoptosis as well as cell growth. We compared here the behaviour of rounded Swiss 3T3 cells aggregated on a cellulose cuprophan membrane to those cultured on dish polystyrene. We demonstrated that cells aggregated on cellulose substrates for up to 48 h underwent programmed cell death that was associated with phosphatidylserine flipping and caspase 9 and caspase 3 activation, suggesting a mitochondria‐dependent apoptotic process. In addition, we found that this phenomenon cannot be entirely explained by disengagement of α5β1 integrin ligation.