Premium
Nitric oxide differentially regulates pro‐ and anti‐angiogenic markers in DLD‐1 colon carcinoma cells
Author(s) -
Hellmuth Markus,
Paulukat Jens,
Ninic Raiko,
Pfeilschifter Josef,
Mühl Heiko
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00275-3
Subject(s) - angiogenesis , downregulation and upregulation , monokine , cancer research , chemokine , chemistry , vascular endothelial growth factor , nitric oxide , interferon , nitric oxide synthase , biology , immunology , endocrinology , immune system , vegf receptors , biochemistry , gene
Inducible nitric oxide (NO) synthase (iNOS) appears to be a marker of tumor progression in colon carcinogenesis. Here we investigated effects of NO on selected chemokines that differentially regulate angiogenesis, namely pro‐angiogenic interleukin (IL)‐8 as well as tumor‐suppressive interferon‐inducible protein‐10 (IP‐10) and monokine induced by interferon‐γ (MIG). These chemokines are expressed by DLD‐1 colon carcinoma cells after stimulation with IL‐1β/interferon‐γ. Expression of IL‐8 was markedly upregulated by NO. Moreover, NO enhanced expression of vascular endothelial growth factor (VEGF). In contrast, expression of IP‐10 and MIG was suppressed by NO. The present data are consistent with previous observations that link NO to enhanced tumor angiogenesis and imply that NO‐mediated upregulation of IL‐8 and VEGF as well as downregulation of IP‐10 and MIG may contribute to this phenomenon.