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Analysis of GT‐3a identifies a distinct subgroup of trihelix DNA‐binding transcription factors in Arabidopsis
Author(s) -
Ayadi Mira,
Delaporte Virginie,
Li You-Fang,
Zhou Dao-Xiu
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00222-4
Subject(s) - promoter , transcription factor , gene , arabidopsis , dna , biology , genetics , transcription (linguistics) , dna binding protein , binding site , regulatory sequence , microbiology and biotechnology , mutant , gene expression , linguistics , philosophy
Trihelix DNA‐binding factors (or GT factors) bind to GT elements found in the promoters of many plant genes. Although the binding specificity and the transcriptional activity of some members (e.g. GT‐1 and GT‐2) have been studied, the regulatory function of this family of transcription factors remains largely unknown. In this work, we have characterised a new GT factor, namely GT‐3a, and a closely related member, GT‐3b. We show that (1) they can form either homo‐ or heterodimers but do not interact with GT‐1; (2) they are predominantly expressed in floral buds and roots; (3) GT‐3a cannot bind to the binding sites of GT‐1 or GT‐2, but binds to the cab2 and rbcS‐1A gene promoters via the 5′‐GTTAC sequence, which has been previously shown to be the core of the Site 1 type of GT elements. These results suggest that GT‐3a and GT‐3b belong to a distinct subgroup of GT factors and that each subgroup of GT factors binds to a functionally distinct type of cis ‐acting GT elements.