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LRP‐1/TβR‐V mediates TGF‐β1‐induced growth inhibition in CHO cells
Author(s) -
Tseng Wen-Fang,
Huang Shuan Shian,
Huang Jung San
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00185-1
Subject(s) - chinese hamster ovary cell , transfection , transforming growth factor , microbiology and biotechnology , wild type , growth inhibition , mutant , receptor , chemistry , cell culture , cell growth , biology , biochemistry , gene , genetics
The type V transforming growth factor‐β (TGF‐β) receptor (TβR‐V) is hypothesized to be involved in cellular growth inhibition by TGF‐β 1 . Recently, TβR‐V was found to be identical to low density lipoprotein receptor‐related protein‐1 (LRP‐1). Here we demonstrate that TGF‐β 1 inhibits growth of wild‐type CHO cells but not LRP‐1‐deficient mutant cells (CHO‐LRP‐1 − cells). Stable transfection of CHO‐LRP‐1 − cells with LRP‐1 cDNA restores the wild‐type morphology and the sensitivity to growth inhibition by TGF‐β 1 . In addition, overexpression of LRP‐1 minireceptors exerts a dominant negative effect and attenuates the growth inhibitory response to TGF‐β 1 in wild‐type CHO cells. These results suggest that LRP‐1/TβR‐V is critical for TGF‐β 1 ‐mediated growth inhibition in CHO cells.