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The nitration of τ protein in neurone‐like PC12 cells
Author(s) -
Cappelletti Graziella,
Tedeschi Gabriella,
Maggioni Maria G.,
Negri Armando,
nis Simona,
Maci Rosalba
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00173-5
Subject(s) - nitration , nitric oxide , microbiology and biotechnology , microtubule , tyrosine , chemistry , cytoskeleton , tubulin , cell , biochemistry , biology , organic chemistry
Tyrosine nitration of proteins is emerging as a post‐translational modification playing a role in physiological conditions. Looking for the molecular events triggered by nitric oxide in nerve growth factor‐induced neuronal differentiation, we now find that nitration occurs on the microtubule‐associated protein τ. In differentiated PC12 cells, we have identified as τ a nitrated protein that co‐immunoprecipitates with α‐tubulin and indicated that the modified protein is associated with the cytoskeleton but it is confined to a restricted cell region. This paper supplies the first evidence that nitration of τ occurs in a physiological process and suggests that it could play a role in neuronal differentiation.