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Effect of hyperosmotic shock on phosphoenolpyruvate carboxykinase gene expression and gluconeogenic activity in the crab muscle
Author(s) -
Schein Vanessa,
Waché Yann,
Etges Rodrigo,
Kucharski Luiz Carlos,
van Wormhoudt Alain,
Da Silva Roselis S.M
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00162-0
Subject(s) - phosphoenolpyruvate carboxykinase , gluconeogenesis , pyruvate kinase , hepatopancreas , biochemistry , biology , amino acid , protein kinase a , kinase , enzyme , microbiology and biotechnology , glycolysis
Chasmagnathus granulata phosphoenolpyruvate carboxykinase (PEPCK) cDNA from jaw muscle was cloned and sequenced, showing a specific domain to bind phosphoenolpyruvate in addition to the kinase‐1 and kinase‐2 motifs to bind guanosine triphosphate (GTP) and Mg 2+ , respectively, specific for all PEPCKs. In the kinase‐1 motifs the GK was changed to RK. The first 19 amino acids of the putative enzyme contain hydrophobic amino acids and hydroxylated residues specific to a mitochondrial type signal. The PEPCK is expressed in hepatopancreas, muscles, nervous system, heart, and gills. Hyperosmotic stress for 24 h increased the PEPCK mRNA level, gluconeogenic and PEPCK activities in muscle.