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Dietary fish oil up‐regulates cholesterol 7α‐hydroxylase mRNA in mouse liver leading to an increase in bile acid and cholesterol excretion
Author(s) -
Bérard Annie M.,
Dumon Marie-France,
Darmon Michel
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00049-3
Subject(s) - cholesterol , excretion , bile acid , cholesterol 7 alpha hydroxylase , medicine , chemistry , fish <actinopterygii> , endocrinology , dietary cholesterol , fish oil , messenger rna , biochemistry , biology , fishery , gene
To investigate the molecular events controlling reverse cholesterol transport, we compared gene expression of normal mouse liver to that of mice fed a long chain (LC) ω‐3 fatty acid‐enriched diet. Using cDNA microarrays, we assessed expression levels of 1176 genes, and we found that D‐site binding protein (DBP) was three‐fold increased in mice on a LC ω‐3 fatty acid‐rich diet compared to controls. DBP is known to increase transcriptional level of cholesterol 7α‐hydroxylase (C7α), the rate‐limiting enzyme for bile acid production and cholesterol excretion, and we found that C7α mRNA was also up‐regulated by LC ω‐3 fatty acids. Moreover, liver X receptor‐α, another transcription factor up‐regulating C7α, was three‐ to four‐fold increased in liver of treated mice. On the other hand, we demonstrated that bile acid and cholesterol excretion were two‐fold increased. These results show that LC ω‐3 fatty acids control cholesterol metabolism in mice at a new endpoint.