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Apg‐2 has a chaperone‐like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas
Author(s) -
Gotoh Kazuhisa,
oguchi Kohsuke,
Higashitsuji Hiroaki,
Kaneko Yoshiyuki,
Sakurai Toshiharu,
Sumitomo Yasuhiko,
Itoh Katsuhiko,
Subjeck John R,
Fujita Jun
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00034-1
Subject(s) - hccs , hepatocellular carcinoma , chaperone (clinical) , biology , gene , microbiology and biotechnology , hsp70 , complementary dna , heat shock protein , luciferase , cancer research , transfection , genetics , medicine , pathology
Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. We constructed subtracted cDNA libraries enriched with genes overexpressed in HCCs. Among the 17 genes identified were molecular chaperones, Hsp110, Hsp90B, and Hsp70‐1. Expression of the Hsp110 family members was further analyzed, and increased transcript levels of Hsp110 and Apg‐2, but not Apg‐1, were found in 12 and 14, respectively, of 18 HCCs. Immunohistochemical analysis demonstrated the overexpression of the proteins in tumor cells. Apg‐2 had chaperone ability similar to Hsp110 in a thermal denaturation assay using luciferase, and showed anti‐apoptotic activity. These results suggest that the Hsp110 family members play important roles in hepatocarcinogenesis through their chaperoning activities.