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Volume‐regulated Cl − channels in human pleural mesothelioma cells
Author(s) -
Meyer Giuliano,
Rodighiero Simona,
Guizzardi Fabiana,
Bazzini Claudia,
Bottà Guido,
Bertocchi Cristina,
Garavaglia Lisa,
Dossena Silvia,
Manfredi Rosangela,
Sironi Chiara,
Catania Anna,
Paulmichl Markus
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00020-1
Subject(s) - mesothelium , western blot , chemistry , cytosol , extracellular , mesothelial cell , mesothelioma , patch clamp , biophysics , ion channel , immunocytochemistry , membrane potential , membrane , microbiology and biotechnology , biochemistry , biology , enzyme , pathology , medicine , endocrinology , receptor , gene
Anion channels in human mesothelial and mesothelioma cell lines were characterized by patch‐clamp and biomolecular approaches. We found an outwardly rectifying anionic current which was inactivated at positive voltages and inhibited by extracellular adenosine 5′‐triphosphate (ATP). Mesothelial and mesothelioma cells behaved differently concerning current inactivation properties. Inactivation is more pronounced and has a steeper onset in mesothelial cells. Different reversal potentials, in asymmetrical Cl − solutions, that could be attributed to a different selectivity of the channel, have been observed in the two cell lines. Mesothelioma cell single‐channel analysis indicates that the number of the same active anion channel (3–4 pS) increased under hypoosmotic conditions. Immunocytochemistry experiments showed the presence of ICln protein in the cytosol and in the plasma membrane. Western blot analysis revealed an increase of ICln in the membrane under hypotonic conditions, an event possibly related to the activation of Cl − channels.