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Absolute expression values for mouse transcripts: re‐annotation of the READ expression database by the use of CAGE and EST sequence tags
Author(s) -
Kodzius Rimantas,
Matsumura Yonehiro,
Kasukawa Takeya,
Shimokawa Kazuro,
Fukuda Shiro,
Shiraki Toshiyuki,
Nakamura Mari,
Arakawa Takahiro,
Sasaki Daisuke,
Kawai Jun,
Harbers Matthias,
Carninci Piero,
Hayashizaki Yoshihide
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(04)00018-3
Subject(s) - serial analysis of gene expression , gene expression , database , expression (computer science) , gene , annotation , biology , sequence (biology) , gene expression profiling , microbiology and biotechnology , genetics , computer science , programming language
The RIKEN expression array database (READ) provides comprehensive gene expression data for the mouse, which were obtained as relative values from microarray double-staining experiments with E17.5 mRNA as common reference. To assign absolute expression values for mouse transcripts within READ, we applied the E17.5 reference sample to CAGE (cap analysis of gene expression) and expressed sequence tag (EST) high-throughput tag sequencing. Newly assigned values within the READ database were validated by comparison to expression data from serial analysis of gene expression, CAGE and EST experiments. These experiments confirmed the great significance of the absolute expression values within the improved READ database. The new Absolute READ database on absolute expression data is available under.