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PPARγ mediates NSAIDs‐induced upregulation of TFF2 expression in gastric epithelial cells
Author(s) -
Shimada Tadahito,
Koitabashi Ayako,
Fujii Yoichiro,
Hashimoto Takashi,
Hosaka Kumi,
Tabei Kyoko,
Namatame Takashi,
Yoneda Masashi,
Hiraishi Hideyuki,
Terano Akira
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01527-8
Subject(s) - downregulation and upregulation , peroxisome proliferator activated receptor , gastric mucosa , reverse transcription polymerase chain reaction , aspirin , receptor , chemistry , transcription factor , gene expression , pharmacology , biology , gene , biochemistry , stomach
Trefoil factor family (TFF) is a group of peptides that play critical roles in maintaining gastric mucosal integrity. In real‐time quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) and reporter gene assays, we show that indomethacin and aspirin upregulate TFF2 expression in MKN45 gastric cells. These drugs also activated peroxisome proliferator‐activated receptor γ (PPARγ) at concentration ranges that increase TFF2 expression, and upregulated TFF2 expression was suppressed by GW9662, a specific inhibitor of PPARγ. These results suggest that indomethacin and aspirin upregulate gastric expression of TFF2 through activation of PPARγ. This mechanism may be important in reducing the extent of gastric mucosal injury caused by the administration of non‐steroidal anti‐inflammatory drugs (NSAIDs).