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Histidine‐containing phosphotransfer domain extinction by RNA interference turns off a cytokinin signalling circuitry in Catharanthus roseus suspension cells
Author(s) -
Papon Nicolas,
Vansiri Amerin,
Gantet Pascal,
Chénieux Jean-Claude,
Rideau Marc,
Crèche Joël
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01522-9
Subject(s) - rna interference , catharanthus roseus , biology , microbiology and biotechnology , gene , intron , hedgehog signaling pathway , transcription (linguistics) , response regulator , rna , signal transduction , genetics , biochemistry , mutant , linguistics , philosophy
We previously reported that cytokinins (CK) induce the fast and specific transcription of CrRR1 , a gene encoding a type A response regulator in Catharanthus roseus cell cultures. Here, we characterized the CrHPt1 gene that encodes a histidine‐containing phosphotransfer domain. CrHPt1 was silenced through RNA interference (RNAi) to test its possible implication in the CK signalling pathway. In transgenic lines stably transformed with an intron‐spliced construct, the degradation of CrHPt1 transcripts abolishes the CK inductive effect on CrRR1 transcription. These result give a new in vivo functional argument for the crucial role of HPt proteins in the CK signalling pathway leading to the expression of the genes encoding type A response regulators. They also show that RNAi is a powerful strategy to turn off the CK signalling circuitry.