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Increased transcription of mitochondrial genes for Complex I in human platelets during ageing
Author(s) -
Merlo Pich Milena,
Raule Nicola,
Catani Lucia,
Fagioli Maria Elena,
Faenza Irene,
Cocco Lucio,
Lenaz Giorgio
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01520-5
Subject(s) - ageing , cytochrome c oxidase , microbiology and biotechnology , gene , platelet , messenger rna , biology , mitochondrion , nad+ kinase , reverse transcription polymerase chain reaction , real time polymerase chain reaction , gene expression , transcription (linguistics) , enzyme , biochemistry , genetics , immunology , linguistics , philosophy
We studied the effect of ageing on the mRNA levels of mitochondria‐encoded polypeptides in human platelets. We used quantitative real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR) to investigate the expression of selected cytochrome c oxidase (COX) genes (subunits I and III) and Complex I genes (subunits reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase (ND)1 and ND5) in platelets from young and aged healthy subjects. Northern blot analysis confirmed the PCR results. COX I expression is higher than that of COX III in both young and aged platelets. A significant increase of transcripts for Complex I was found during ageing. On the contrary, the mRNA levels of the two COX subunits did not significantly vary during ageing.