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Identification of a novel protein complex containing annexin A4, rabphilin and synaptotagmin
Author(s) -
Willshaw Angela,
Grant Karen,
Yan Jun,
Rockliffe Nichola,
Ambavarapu Sailaja,
Burdyga Galina,
Varro Andrea,
Fukuoka Shin-Ichi,
Gawler Debra
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01513-8
Subject(s) - synaptotagmin 1 , annexin a2 , synaptotagmin i , synaptic vesicle , exocytosis , microbiology and biotechnology , annexin , chemistry , biology , biochemistry , vesicle , secretion , membrane , cell
Rabphilin is a synaptic vesicle‐associated protein proposed to play a role in regulating neurotransmitter release. Here we report the isolation and identification of a novel protein complex containing rabphilin, annexin A4 and synaptotagmin 1. We show that the rabphilin C2B domain interacts directly with the N‐terminus of annexin A4 and mediates the co‐complexing of these two proteins in PC12 cells. Analyzing the cellular localisation of these co‐complexing proteins we find that annexin A4 is located on synaptic membranes and co‐localises with rabphilin at the plasma membrane in PC12 cells. Given that rabphilin and synaptotagmin are synaptic vesicle proteins involved in neurotransmitter release, the identification of this complex suggests that annexin A4 may play a role in synaptic exocytosis.