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Creatine increases IGF‐I and myogenic regulatory factor mRNA in C 2 C 12 cells
Author(s) -
Louis Magali,
Van Beneden Ronald,
Dehoux Mischaël,
Thissen Jean Paul,
Francaux Marc
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01504-7
Subject(s) - myogenesis , muscle hypertrophy , messenger rna , creatine , medicine , endocrinology , insulin like growth factor , chemistry , growth factor , transcription factor , biology , microbiology and biotechnology , myocyte , gene , biochemistry , receptor
Addition of creatine to the differentiation medium of C 2 C 12 cells leads to hypertrophy of the myotubes. To investigate the implication of insulin‐like growth factor I (IGF‐I) and myogenic regulatory factors (MRFs) in this hypertrophy, their mRNA levels were assessed during the first 72 h of differentiation. Creatine significantly increased the IGF‐I mRNA level over the whole investigated period of time, whereas the MRF mRNA levels were only augmented at precise moments, suggesting a general activation mechanism for IGF‐I and a specifically regulated mechanism for MRF transcription. Our results suggest therefore that creatine‐induced hypertrophy of C 2 C 12 cells is at least partially mediated by overexpression of IGF‐I and MRFs.