Premium
Functional silencing of hepatic microsomal glucose‐6‐phosphatase gene expression in vivo by adenovirus‐mediated delivery of short hairpin RNA
Author(s) -
Huang Alan,
Chen Yan,
Wang Xinzhong,
Zhao Shanchuan,
Su Nancy,
White David W
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01499-6
Subject(s) - small hairpin rna , gene silencing , microbiology and biotechnology , gene expression , in vivo , biology , messenger rna , phosphatase , rna , gene , chemistry , enzyme , biochemistry
An expression cassette containing mouse U6 polymerase III promoter directing expression of short hairpin RNA (shRNA) targeting murine microsomal glucose‐6‐phosphatase (G6P) transcript was generated. This construct was packaged into an adenoviral (AdV) backbone and viral stocks generated. Mice injected intravenously with AdV‐G6PshRNA exhibited a significant reduction in postprandial glucose levels and had significantly elevated steady‐state hepatic glycogen stores. Target gene silencing was confirmed by measurements demonstrating a significant reduction in both hepatic G6P transcript level and phosphohydrolase activity. These findings provide evidence that AdV delivery of expressed shRNA can be a productive tool to explore gene function in vivo.