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Mitochondrial small heat‐shock protein enhances thermotolerance in tobacco plants
Author(s) -
Sanmiya Kazutsuka,
Suzuki Katsumi,
Egawa Yoshinobu,
Shono Mariko
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01494-7
Subject(s) - nicotiana tabacum , lycopersicon , heat shock protein , biology , gene , transgene , genetically modified crops , sense (electronics) , solanaceae , microbiology and biotechnology , mitochondrion , gene expression , botany , genetics , chemistry
To clarify the role of mitochondrial small heat‐shock protein (MT‐sHSP) in the heat‐shock response, we introduced the tomato ( Lycopersicon esculentum ) MT‐sHSP gene under the control of the 35S promoter into tobacco ( Nicotiana tabacum ), and examined the thermotolerance of the transformed plants. Irrespective of the orientation, sense or antisense, of the gene, the transgenic plants exhibited a normal morphology and growth rate in the vegetative growth stage. When 4‐week‐old seedlings were exposed to sudden heat stress, the sense plants which overexpress the MT‐sHSP gene exhibited thermotolerance, whereas the antisense plants in which the expression of the gene is suppressed exhibited susceptibility.