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Enhancement of RNAi activity by improved siRNA duplexes
Author(s) -
Hohjoh Hirohiko
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01492-3
Subject(s) - rna interference , small interfering rna , sense (electronics) , gene , rna , microbiology and biotechnology , rna silencing , gene silencing , trans acting sirna , function (biology) , biology , chemistry , computational biology , genetics
RNA interference (RNAi) is a powerful tool for suppressing the expression of a gene of interest, in which 21–25 nucleotide short interfering RNA (siRNA) duplexes homologous to the silenced gene function as sequence‐specific RNAi mediators. The present study shows that newly designed siRNA duplexes, ‘fork‐siRNA duplexes’, whose sense‐stranded siRNA elements carry one to four nucleotide mismatches at the 3′‐ends against the antisense‐stranded siRNA elements, can enhance RNAi activity over conventional siRNA duplexes in cultured mammalian cells.