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A plant outer mitochondrial membrane protein with high amino acid sequence identity to a chloroplast protein import receptor
Author(s) -
Chew Orinda,
Lister Ryan,
Qbadou Soumya,
Heazlewood Joshua L,
Soll Jurgen,
Schleiff Enrico,
Millar A.Harvey,
Whelan James
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01457-1
Subject(s) - translocase of the outer membrane , biology , translocase , arabidopsis thaliana , protein targeting , translocase of the inner membrane , mitochondrion , arabidopsis , bacterial outer membrane , biochemistry , chloroplast , vesicle associated membrane protein 8 , mitochondrial membrane transport protein , hspa9 , mitochondrial carrier , inner mitochondrial membrane , microbiology and biotechnology , membrane protein , peptide sequence , gene , membrane , chromosomal translocation , escherichia coli , mutant
We have identified a novel protein on the outer membrane of Arabidopsis thaliana mitochondria. This protein displays 67% sequence identity with the 64 kDa translocase of the outer envelope membrane of chloroplasts (Toc). A mitochondrial localisation for this protein was determined by (i) its presence in the proteome of highly purified Arabidopsis mitochondria, (ii) Western blot analysis with antibodies to Toc64 from pea that indicate its presence in Arabidopsis and pea mitochondria, (iii) green fluorescent protein fusion proteins that indicate an exclusive mitochondrial localisation for this protein, and (iv) expression profiles in various tissue types and during development that are more similar to translocase of the outer mitochondrial membrane components than to chloroplastic Toc components. Thus Arabidopsis mitochondria contain a protein with high sequence identity to a plastid protein import receptor.