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AtPNP‐A is a systemically mobile natriuretic peptide immunoanalogue with a role in Arabidopsis thaliana cell volume regulation
Author(s) -
Morse Monique,
Pironcheva Ganka,
Gehring Chris
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01384-x
Subject(s) - natriuretic peptide , recombinant dna , peptide , npr2 , signal peptide , microbiology and biotechnology , arabidopsis thaliana , npr1 , arabidopsis , biology , function (biology) , homeostasis , chemistry , biochemistry , medicine , gene , heart failure , mutant
Cellular and physiological evidence suggests the presence of a novel class of systemically mobile plant molecules that are recognized by antibodies against vertebrate atrial natriuretic peptides (ANPs). In order to characterize the function of these immunoanalogues we have expressed the full‐length recombinant (AtPNP‐A[1–126]) and demonstrate that this molecule induces osmoticum‐dependent H 2 O uptake into protoplasts at nanomolar concentrations and thus affects cell volume. A similar response is also seen with a recombinant that does not contain the signal peptide (AtPNP‐A[26–126]) as well as a short domain (AtPNP‐A[33–66]) that shows homology to the vertebrate peptide. Taken together, these findings suggest that AtPNP‐A has an important and systemic role in plant growth and homeostasis.